MATERIALS

Reagents:

1. 5% Methanol
2. 1% Formic Acid
3. Acetonitrile
4. HPLC Grade Water
5. 6 M Urea Solution
6. Trypsin Digest
7. Promega Sequencing Grade Modified Trypsin
8. Dithiothreitol
9. Dimethyl Sulfoxide
10. Iodoacetamide
11. Liquid Nitrogen
12. Extraction Buffer (0.3 M KCl, 0.15 M KH2PO4, 0.15 M K2HPO4 at pH 6.5)

Tubes:

1. Corning 15 mL Plastic Centrifuge Tube
2. Corning 50 mL Plastic Centrifuge Tube
3. 1.5 mL Eppendorf Reaction Tube

Equipment

1. Thermoshaker
2. Fritsch Pulverisette 14 Motor Mill with 2 mm Sieve
3. GFL 3005 Rotary Lab Shaker
4. Phenomenex Strata-X 33u Polymeric Reversed-Phase Cartridges
5. S-Concentrator BA-VC-300H
6. LTQ Orbitrap XL
7. AB SCIEX TripleTOF 5600
8. Accela HPLC
9. Eksigent nanoLC
10. VWR Hitachi HPLC
11. Turbo V Electrospray Ionization Source
12. Eksigent Ekspert MicroLC 200 System
13. Phenomenex Kinetex C18 (2.6 μm, 100 mm × 2.10 mm) column
14. Thermo Scientific C18 (2.0 μm, 250 mm × 75 μm) column
15. Agilent ZORBAX XDB C18 (3.5 μm, 0.5 × 150 mm) column
16. Eksigent HALO C18 (2.7 μm, 0.5 × 100 mm) column