Thursday, 6 February 2014

INTRODUCTION





The detection of horse/porcine meat in halal beef is an important aspect in food quality control as it concerns consumers against certain species of animals such as horse/porcine. The accidental or fraudulent contamination of horse/porcine meat in halal beef is an issue which many scientific and religious institutions have started tackling. Some common analytical methods to detect horse/porcine meat in halal beef are two dimensional sodium dodecyl sulfate - polyacrylamide electrophoresis (2D SDS-PAGE), polymerase chain reaction (PCR), enzyme-linked immunoabsorbent assay (ELISA) and mass spectrometry (MS). An alternative approach as identified by the researchers is the utilization of tandem mass spectrometry (TMS) as a more efficient method in detecting horse/porcine meat in halal beef. The effectiveness of the current analytical methods versus the method in question is determined using the limit of detection (LOD).

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